Testing Services

Testing Services

Over the years, Bhavan's Research Center for Microbiology has systematically leveraged its core competency in microbiology through partnerships and alliances, to move up the value chain in analysis. It is endowed with a rich pool of experienced microbiologists acting as drivers of growth, to raise the laboratory’s capabilities to global standards. Besides routine Bio-burden and Pathogen testing of Food, Water, Cosmetics, Personal Care and Environmental samples, it undertakes preservative efficacy testing and antimicrobial studies using AOAC, ISO, EN, ASTM protocols as well as customer specified protocols.

It has developed good quality management systems in the lab along with a very strong culture maintenance system in line with international specifications, with all the tests being performed within 5 passages from procured culture strains.

Following are the tests under ISO/IEC 17025:2005 (NABL) accreditation



# Products/Material of Test Specific tests performed Test methods/Standards against which tests are performed
1 Drinking water Coliforms IS: 1622: 1981 RA 2014
E. coli IS: 1622: 1981 RA 2014
2 Packaged Drinking Water/ Packaged Natural Mineral Water Coliforms IS: 15185:2016
E. coli IS: 15185:2016
Fecal streptococci IS: 15186:2002 RA 2014
Total Viable Colony Count @ 22°C for 72hrs IS 5402: 2012
Total Viable Colony Count @ 37°C for 24hrs IS 5402: 2012
Pseudomonas aeruginosa IS: 13428: 2005 RA 2014: Annex D
Salmonella spp IS: 15187: 2016
Shigella spp IS: 5887 (Part 7): 1999 RA 2013
S. aureus IS:5887(Part 2) :1976 RA 2013
Sulphite Reducing Anaerobes IS: 13428: 2005 RA 2014: Annex C
Vibrio parahaemolyticus IS 5887 (Part 5) 1976 RA 2013
Yeast and Mold Count IS 5403: 1999 RA 2013
3 Water for processed food industry Coliforms IS: 1622: 1981 RA 2014
Standard Plate Count IS: 1622: 1981 RA 2014
Proteolytic Organisms IS: 4251: 1967 RA 2015
Lipolytic Organisms IS: 4251: 1967 RA 2015

Biological Tests on other Miscellaneous Test Items

1 Water Purifier Evaluation of drinking water treatment technology for microbiological reduction performance - Viruses - MS 2 Bacteriophage National Sanitary Foundation (NSF) P248: 2008
Evaluation of drinking water treatment technology for microbiological reduction performance - Microspheres National Sanitary Foundation (NSF)/ ANSI 53: 2011a / P231: 2003
Evaluation of drinking water treatment technology for microbiological reduction performance - Bacteria - Raoultella (Klebsiella) terrigena National Sanitary Foundation (NSF) P231: 2003/ P248: 2008

# Products/Material of Test Specific tests performed Test methods/Standards against which tests are performed
1 Cosmetics & Cosmetic Raw Materials Total Microbial Count IS: 14648:2011
Gram Negative Enteric Pathogens IS: 14648:2011
Staphylococcus aureus IS: 14648:2011
Pseudomonas aeruginosa IS: 14648:2011
Candida albicans IS: 14648:2011
Effectiveness of Antimicrobial Preservative / Challenge Test AOAC Official Method of Analysis 998.10

Standard test method for assessment of antimicrobial activity for water miscible compounds using Time - Kill Procedure.

ASTM: E2783- 11

Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics.

BS EN 1040:2005: E

# Products/Material of Test Specific tests performed Test methods/Standards against which tests are performed
1 Microbiological Monitoring In Air/Surface Area Microbial count in surface by swab test In House method - FS 004 BRC
In House method - CS 002 BRC
Microbial count in air by settle plate method Sedimentation method
In House method - FA 002 BRC
In House method - CA 001 BRC

# Products/Material of Test Specific tests performed Test methods/Standards against which tests are performed
1 Antiseptics/Sanitizers

Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics

BS EN 1040:2005: E

Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas

EN 1276: 2009 E

Quantitative non porous surface test for the evaluation of bactericidal and / or fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas

BS EN 13697: 2015

Standard test method for assessment of antimicrobial activity for water miscible compounds using Time - Kill Procedure

ASTM: E2783 - 11
Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas (Bacillus subtilis ATCC 6633) EN 13704: 2002 E
Turbidometric or Tube assay Method IP 2014, Volume I, chapter No. 2.2 Biological methods
Disc diffusion susceptibility test of Bauer Kirby IP 2014, Volume I, chapter No. 2.2 Biological methods
Cup plate Method IP 2014, Volume I, chapter No. 2.2 Biological methods
Phenol Coefficient Method- Testing disinfectants against Staphylococcus aureus ATCC 6538 AOAC 2011, Official Method of Analysis 955.12
Phenol Coefficient Method- Testing disinfectants against Pseudomonas aeruginosa ATCC 15442 AOAC 2011, Official Method of Analysis 955.13